Δ 9-tetrahydrocannbinol (THC) in the range of ng/mg of hair or lower, and THCCOOH with pg/mg concentrations. This is especially relevant for cannabinoids that have low concentrations in hair, i.e. The confirmation cutoff concentration has not been finalized but will be either 0.05 or 0.1 pg 11-nor-9-carboxy-Δ 9-tetrahydrocannbinol (THCCOOH)/mg hair.Īn important issue is utilization of sufficiently sensitive and specific analytical procedures to detect and quantify drugs in hair. If implemented, the guidelines will establish an initial test cutoff concentration of 1 pg marijuana metabolites/mg hair. Guidelines for workplace hair testing have been proposed by the Substance Abuse Mental Health Services Administration in the United States. Workplace programs include hair testing due to the ease of collection, difficulty of adulteration and longer detection times. verifying drug use history, identifying drug facilitated sexual assault, proving drug use in child custody cases, monitoring abstinence of parolees, drug treatment participants or employees, and documenting in utero exposure. Applications of hair testing include criminal investigations, i.e. In addition, many drugs are well preserved in hair, the extreme example being cocaine detected in the hair of a 900-year old mummy. One advantage of hair testing is its larger window of drug detection as compared to other biological fluids. Testing hair for drugs of abuse was introduced over 50 years ago. Using an immunoassay cutoff concentration of 5 pg THC equivalents/mg hair, 83% of specimens that screened positive were confirmed by GCMSMS at a cutoff concentration of 0.1 pg THCCOOH/mg hair. THC and THCCOOH concentrations were positively correlated (r = 0.38, p < 0.01, Pearson's product moment correlation). For specimens with detectable cannabinoids, concentrations ranged from 3.4 to > 100 pg THC/mg and 0.10 to 7.3 pg THCCOOH/mg hair. Detection rates were significantly different (p 0.3, Fisher's exact test). Two specimens (3.8%) had measurable THC only, 14 (26%) THCCOOH only, and 18 (34%) both cannabinoids. Nineteen specimens (36%) had no detectable THC or 11-nor-9-carboxy-THC (THCCOOH) at the GCMSMS limits of quantification (LOQ) of 1.0 and 0.1 pg/mg hair, respectively. Pre- and post dose detection rates did not differ statistically, therefore, all 53 specimens were considered as one group for further comparisons. Cannabinoid concentrations in all hair specimens were determined by ELISA and GCMSMS. Additional hair specimens were collected following smoking of two 2.7% THC cigarettes (N = 13) or multiple oral doses totaling 116 mg THC (N = 2). Drug use was also documented by a positive cannabinoid urinalysis, a hair specimen was collected from each subject and they were admitted to a closed research unit. 1 to 5 cannabis cigarettes per week, (N = 20). The subjects completed a questionnaire indicating daily cannabis use (N = 18) or non-daily use, i.e. Fifty-three head hair specimens were collected from 38 males with a history of cannabis use documented by questionnaire, urinalysis and controlled, double blind administration of Δ 9-tetrahydrocannabinol (THC) in an institutional review board approved protocol.
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